Electron Transport Systems of Lung Microsomes and Their Physiological Functions: I. Intracellular Distribution of Oxidative Enzymes in Lung Cells
- 1 December 1971
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 70 (6) , 981-991
- https://doi.org/10.1093/oxfordjournals.jbchem.a129728
Abstract
Rabbit lung cells were homogenized in 0.15 m KCl containing 10 mM phosphate buffer, pH 7.4, and the homogenate was fractionated by differential centrifugation. The distribution patterns of chemical components and oxidative enzymes in lung subcel-lular fractions were similar to those in liver cells. Lung mitochondria (Fraction II) showed a cytochrome pattern characteristic for the mitochondrial respiratory chain, but the contents of cytochromes were only about one fifth those in liver mitochondria. Lung microsomes (Fraction III) contained cytochromes b5 and P-450 as well as NADH-and NADPH-cytochrome c reductase activities. The contents of the cytochromes and the specific activities of the reductases were, however, one third to one fifth the levels reported for liver microsomes. The NADH-reduced minus oxidized difference spectrum of lung microsomes, representing the difference spectrum of cytochrome b5 was slightly different from that of liver microsomes.Keywords
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