Cocal strain of vesicular stomatitis virus was propagated in Aedes albopictus and Aedes aegypti cell lines. Infection of the cell lines at 30 °C resulted in a low-titered virus infection involving only a small proportion of the cell population with no cytopathic effects. Virus titers in A. albopictus were increased by adsorption with DEAE-dextran or by increasing the temperature of incubation. Use of a high-input multiplicity of virus and incubation of cells at 37 °C resulted in 100% infection of cells, allowing an electron-microscopic study of virus development. These studies indicated that virus maturation in A. albopictus cells occurred primarily at intracytoplasmic vesicles.