Intracellular Virus-like Particles in Lentinus edodes

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Abstract
Polyhedral virus-like particles, about 39 nm in diameter (39 nm VLP), were studied by EM in thin sections of L. edodes (Berk.) Sing. monokaryon. They were common in the cytoplasm and in vacuoles of the hyphal cells, either irregularly scattered or in aggregates which were sometimes crystalline especially in old mycelium. Crystals were found almost exclusively within cytoplasm and vacuoles in disintegrating cells. Examination of fused monokaryons suggests that the cytoplasmic 39 nm VLP may move from one cell to another through the breakdown of a dolipore septum during dikaryotization.