Deciphering the fluorescent variability of human genomic heterochromatin by DA/DAPI technique
- 28 June 2008
- journal article
- Published by Wiley in Clinical Genetics
- Vol. 42 (5) , 267-270
- https://doi.org/10.1111/j.1399-0004.1992.tb03253.x
Abstract
A number of selective staining techniques have been utilized to decipher the variability of pericentromeric heterochromatin. One such technique is called DA/DAPI and it is believed to be stain specific. However, we demonstrate otherwise and suggest that pericentromeric regions of all human chromosomes stain positive by DA/DAPI-technique. It must be emphasized that the incidence of DA/DAPI positive stained chromosomes, other than 1, 9, 15, 16 and Y, is a rare occurrence and only a small portion of the pericentromeric region is DA/DAPI positive, as reported here using 50 normal individuals.Keywords
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