Dephosphorylation of distinct sites on microtubule‐associated protein MAP1B by protein phosphatases 1, 2A and 2B
- 6 September 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 330 (1) , 85-89
- https://doi.org/10.1016/0014-5793(93)80925-k
Abstract
Rat brain microtubule-associated protein MAP1 B has been tested as a substrate for Ser/Thr protein phosphatases (PP). The dephosphorylation reactions were followed by specific antibodies recognizing phosphorylated and phosphorylatable epitopes. One set of phosphorylation sites on MAPI B are referred to as mode I sites, and their phosphorylation is presumably catalyzed by proline-directed protein kinases. These mode I sites are efficiently dephosphorylated by PP2B and 2A but not by PP1. Another set of phosphorylation sites on MAP1B are named mode II sites, and their phosphorylation is possibly due to casein kinase II. These mode II sites are dephosphorylated by PP2A and PP1, the PP2B being ineffective. The selectivity of phosphatases for different sites within the same protein indicates the complexity of the dephosphorylation reactions regulating the functionality of MAP1B in neurons.Keywords
This publication has 35 references indexed in Scilit:
- The regulation and function of protein phosphatases in the brainMolecular Neurobiology, 1991
- Association of microtubule-associated protein (MAP1B) with growing axons in cultured hippocampal neuronsMolecular and Cellular Neuroscience, 1991
- Immunochemical evidence that fragments of phosphorylated MAP5 (MAP1B) are bound to neurofibrillary tangles in Alzheimer's diseaseNeuron, 1990
- A casein kinase II-related activity is involved in phosphorylation of microtubule-associated protein MAP-1B during neuroblastoma cell differentiation.The Journal of cell biology, 1988
- Protein phosphatases active on acetyl-CoA carboxylase phosphorylated by casein kinase I, casein kinase II and the cAMP-dependent protein kinaseBiochemical and Biophysical Research Communications, 1985
- A polymer-dependent increase in phosphorylation of beta-tubulin accompanies differentiation of a mouse neuroblastoma cell line.The Journal of cell biology, 1985
- Calcineurin is a calmodulin-dependent protein phosphataseBiochemical and Biophysical Research Communications, 1982
- Ca2+-induced hydrophobic site on calmodulin: Application for purification of calmodulin by phenyl-Sepharose affinity chromatographyBiochemical and Biophysical Research Communications, 1982
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970