Insights into Translational Termination from the Structure of RF2 Bound to the Ribosome
- 7 November 2008
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 322 (5903) , 953-956
- https://doi.org/10.1126/science.1164840
Abstract
The termination of protein synthesis occurs through the specific recognition of a stop codon in the A site of the ribosome by a release factor (RF), which then catalyzes the hydrolysis of the nascent protein chain from the P-site transfer RNA. Here we present, at a resolution of 3.5 angstroms, the crystal structure of RF2 in complex with its cognate UGA stop codon in the 70S ribosome. The structure provides insight into how RF2 specifically recognizes the stop codon; it also suggests a model for the role of a universally conserved GGQ motif in the catalysis of peptide release.Keywords
This publication has 31 references indexed in Scilit:
- Structural basis for translation termination on the 70S ribosomeNature, 2008
- Peptide release on the ribosome depends critically on the 2′ OH of the peptidyl–tRNA substrateRNA, 2008
- Pseudouridylation of helix 69 of 23S rRNA is necessary for an effective translation terminationProceedings of the National Academy of Sciences, 2007
- Two Distinct Components of Release Factor Function Uncovered by Nucleophile Partitioning AnalysisMolecular Cell, 2007
- An intact ribose moiety at A2602 of 23S rRNA is key to trigger peptidyl-tRNA hydrolysis during translation terminationNucleic Acids Research, 2007
- Release Factors 2 from Escherichia coli and Thermus thermophilus: structural, spectroscopic and microcalorimetric studiesNucleic Acids Research, 2007
- Structure of the 70 S Ribosome Complexed with mRNA and tRNAScience, 2006
- Crystal Structures of the Ribosome in Complex with Release Factors RF1 and RF2 Bound to a Cognate Stop CodonCell, 2005
- Structure of the Escherichia coli ribosomal termination complex with release factor 2Nature, 2003
- Release of Peptide Promoted by the GGQ Motif of Class 1 Release Factors Regulates the GTPase Activity of RF3Molecular Cell, 2002