Demonstration of a tandem pair of complement protein modules in GABAB receptor 1a
- 7 August 1998
- journal article
- Published by Wiley in FEBS Letters
- Vol. 432 (3) , 103-108
- https://doi.org/10.1016/s0014-5793(98)00794-7
Abstract
We have subcloned and expressed the N-terminal portion of the recently sequenced metabotropic GABA receptor, GABABR1a. This region of the receptor contains a complement protein-like amino acid sequence. The purified 140-residue recombinant protein fragment was soluble and stable. Mass spectrometry indicated formation of four disulfide bonds, as expected if two complement protein modules (CPs, also known as SCRs, Sushi domains) are formed. The circular dichroism spectrum was unusual and characteristic of CPs. Differential scanning calorimetry demonstrated a melting point (64°C), and total enthalpy commensurate with two fully folded domains. We thus conclude that the 1a subtype of the GABAB receptor, but not the 1b subtype, contains a pair of CPs and we present a three-dimensional model of this regionKeywords
This publication has 28 references indexed in Scilit:
- NMR studies of a viral protein that mimics the regulators of complement activationJournal of Molecular Biology, 1997
- Expression cloning of GABAB receptors uncovers similarity to metabotropic glutamate receptorsNature, 1997
- GABAB receptors: targets for drug developmentDrug Discovery Today, 1996
- Tissue- and Species-Specific Expression of sp56, a Mouse Sperm Fertilization ProteinScience, 1995
- Comparative Protein Modelling by Satisfaction of Spatial RestraintsJournal of Molecular Biology, 1993
- Solution Structure of a Pair of Complement Modules by Nuclear Magnetic ResonanceJournal of Molecular Biology, 1993
- PROCHECK: a program to check the stereochemical quality of protein structuresJournal of Applied Crystallography, 1993
- hikaru genki, a CNS-specific gene identified by abnormal locomotion in drosophila, encodes a novel type of proteinNeuron, 1993
- Quality control of protein models: directional atomic contact analysisJournal of Applied Crystallography, 1993
- MOLSCRIPT: a program to produce both detailed and schematic plots of protein structuresJournal of Applied Crystallography, 1991