Application of Fingerprinting Techniques to Iodinated Nucleic Acids
- 1 November 1973
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 70 (11) , 3260-3264
- https://doi.org/10.1073/pnas.70.11.3260
Abstract
Several techniques of RNA and DNA finger-printing and determination of sequence have been applied to nucleic acids labeled with (125)I. Fingerprints of human 5S RNA and bacteriophage f2 RNA resemble those of their noniodinated counterparts both in complexity and in specific pattern. Iodination as used here is thus a general labeling procedure, and appears principally to label cytidine residues. This iodination method shows little sensitivity to potential structure in single-stranded RNA molecules, yields stable oligonucleotide products in a reproducible manner, and does not change the specificity of several ribonucleases and deoxyribonucleases.Keywords
This publication has 19 references indexed in Scilit:
- Iodination of nucleic acids in vitroBiochemistry, 1971
- Polypeptide Chain Initiation: Nucleotide Sequences of the Three Ribosomal Binding Sites in Bacteriophage R17 RNANature, 1969
- Chromatography of 32P‐Labelled Oligonucleotides on Thin Layers of DEAE‐CelluloseEuropean Journal of Biochemistry, 1969
- Sequence of a Ribosome Binding Site in Bacteriophage Qβ-RNANature, 1969
- Nucleotide Sequence from the Coat Protein Cistron of R17 Bacteriophage RNANature, 1969
- Use of polynucleotide kinase in fingerprinting non-radioactive nucleic acidsJournal of Molecular Biology, 1969
- Nucleotide Sequence of KB Cell 5 S RNAScience, 1967
- Amber mutants and chain termination in vitroJournal of Molecular Biology, 1967
- Purification and properties of the replicative intermediate of the RNA bacteriophage R17.Proceedings of the National Academy of Sciences, 1966
- A two-dimensional fractionation procedure for radioactive nucleotidesJournal of Molecular Biology, 1965