Triglycerides Are Major Determinants of Cholesterol Esterification/Transfer and HDL Remodeling in Human Plasma
- 1 November 1995
- journal article
- research article
- Published by Wolters Kluwer Health in Arteriosclerosis, Thrombosis, and Vascular Biology
- Vol. 15 (11) , 1819-1828
- https://doi.org/10.1161/01.atv.15.11.1819
Abstract
Lecithin:cholesterol acyltransferase (LCAT) and cholesteryl ester transfer protein (CETP) are responsible for the esterification of cell-derived cholesterol and for the transfer of newly synthesized cholesteryl esters (CE) from HDL to apoB-containing lipoproteins in human plasma. LCAT and CETP are also crucial factors in HDL remodeling, a process by which HDL particles with a high capacity for cell cholesterol uptake are generated in plasma. In the present study, cholesterol esterification and transfer were evaluated in 60 patients with isolated hypercholesterolemia (HC, n=20) and isolated (HTG, n=20) or mixed hypertriglyceridemia (MHTG, n=20) and in 20 normolipidemic healthy individuals (NL). Cholesterol esterification rate (CER) and net CE transfer rate (CETR) were measured in whole plasma. LCAT and CETP concentrations were determined by specific immunoassays. HDL remodeling was analyzed by monitoring changes in HDL particle size distribution during incubation of whole plasma at 37°C. Mean CER ...Keywords
This publication has 58 references indexed in Scilit:
- Differential effect of subspecies of lipoprotein containing apolipoprotein A-I on cholesterol efflux from cholesterol-loaded macrophages: Functional correlation with lecithin : cholesterol acyltransferaseBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1992
- Differential effects of cis and trans fatty acid isomers, oleic and elaidic acids, on the cholesteryl ester transfer protein activityBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1992
- Apolipoprotein A-II modulates HDL remodeling in plasmaBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1992
- Cholesteryl ester transfer protein and hepatic lipase activity promote shedding of apo A-I from HDL and subsequent formation of discoidal HDLBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1992
- Apolipoprotein AIMilano. Partial lecithin: Cholesterol acyltransferase deficiency due to low levels of a functional enzymeBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1990
- High-Density Lipoprotein — The Clinical Implications of Recent StudiesNew England Journal of Medicine, 1989
- Reactivity of HDL subfractions towards lecithin-cholesterol acyltransferase. Modulation by their content in free cholesterolBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1989
- In vitro formation of HDL-2 from HDL-3 and triacylglycerol-rich lipoproteins by the action of lecithin: cholesterol acyltransferase and cholesterol ester transfer proteinBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1987
- Competitive inhibition of plasma cholesterol esterification by human high-density lipoprotein-subfraction 2Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1984
- Interconversion of high density lipoproteins during incubation of human plasmaBiochemical and Biophysical Research Communications, 1981