The use of nurse culture in the development of haploid clones in tomato
- 1 January 1972
- journal article
- Published by Springer Nature in Planta
- Vol. 104 (4) , 357-361
- https://doi.org/10.1007/bf00386318
Abstract
A nurse culture procedure and an appropriate medium are described for obtaining vigorous haploid clones originating from single tomato pollen grains. The grains are plated on filter-paper disks mounted over intact anthers placed on a modified Murashige and Skoog medium. The plating efficiencies of such pollen cells ranges between 0 and 60%. The variability in the plating efficiencies and the potential use of this technique over that of anther culture are discussedKeywords
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