EXPERIMENTAL TUMORS OF MYXOID MALIGNANT FIBROUS HISTIOCYTOMA and HYALURONIC ACID PRODUCTION

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Abstract
Using B-10 tumor cells originated from mouse peritoneal macrophages transformed by simian virus 40, we succeeded in producing tumors in an ascitic form similar to human myxoid malignant fibrous histiocytoma. The tumor cells possessed Fc and C3 receptors, immunophagocytic activity, and lysosomal enzymes. They showed pseudopodic extensions of the cytoplasm containing lysosomes. Therefore, they maintained the functional and morphological characteristics of macrophages. On cellulose acetate electrophoresis with or without enzymatic degradation, th ascitic fluid contained a single component of glycosaminoglycans; hyaluronic acid. Electron microscopy utilizing dialyzed iron demonstrated electron-dense reaction products on the cell surfaces. Thus, the histiocytic origin of malignant fibrous histiocytoma was suggested and possibility was expressed, concerning the histogenesis of myxoid malignant fibrous histiocytoma, that the transformed tumor cells could synthesize hyaluronic acid on the cell surface and release it into the stroma.