An improved silver staining procedure for schizodeme analysis in polyacrylamide gradient gels
Open Access
- 1 March 1990
- journal article
- research article
- Published by FapUNIFESP (SciELO) in Memórias do Instituto Oswaldo Cruz
- Vol. 85 (1) , 101-106
- https://doi.org/10.1590/s0074-02761990000100017
Abstract
A simple protocol is described for the silver staining of polyacrylamide gradient gels used for the separation of restriction fragments of kinetoplast DNA [schizodeme analysis of trypanosomatids (Morel et al., 1980)]. The method overcomes the problems of non-uniform staining and strong background color which are frequently encountered when conventional protocols for silver staining of linear gels are applied to gradient gels. The method described has proven to be of general applicability for DNA, RNA and protein separations in gradient gels.Keywords
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