The Effect of Low Temperatures upon the Viability of Unsporulated Oocysts of Ovine Coccidia

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Abstract
The minimum survival temp, of the unsporulated oocysts of 3 spp. of Eimeria is detd. experimentally to lie between -19 and -25[degree]C. Oocysts stored at -19[degree]C for as long as 60 days are unaffected. Oocysts stored at -25[degree]C for 7 days sporulate normally but such treatment for 14 days results in a significant reduction in viability. The oocysts will tolerate storage at -30[degree]C for 24 hrs. if first conditioned at -19[degree]C. Lower temps, are lethal. A comparison of air and soil temps, at Laramie, Wyoming for 5 winters indicates that the soil surface temp, rarely reaches the extremes of low which occur in the air masses and will not normally be lethal for these 3 spp.

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