POOR RESPONSE OF WV/WX MICE TO A GRAFTED NEUTROPHILIA-INDUCING, "COLONY-STIMULATING-FACTOR-PRODUCING TUMOR

  • 1 January 1984
    • journal article
    • research article
    • Vol. 12  (11) , 850-855
Abstract
Although mice possessing 2 mutant genes at the W locus have a defect in multipotential hematopoietic stem cells that form macroscopic colonies in the spleen of irradiated mice, the number of neurtrophils in the blood of these mutant mice is normal or nearly normal. Neutrophil production was investigated using the NFSA fibrosarcoma of C3H mouse origin, which induces neutrophilia accompanied by production of a neutrophil-macrophage colony-stimulating factor by the tumor. When the NFSA tumor was transplanted to (C57BL/6 .times. C3H/He)F1-Wv/Wx or to congenic +/+ mice, neutrophila developed in mice of both genotypes. There was a significant difference between the degree of neutrophilia that developed in them; there was a 107-fold increase in the +/+ mice, but only a 28-fold increase in the Wv/Wx mice 4 wk after tumor transplantation. Doubly heterozygous W mice have multipotential stem cells with diminished ability to respond to stimulation. The unperturbed condition may not provide a sufficient stimulus to demonstrate the defect in neutrophil production in doubly heterozygous W mutant mice.