Production of Anthocyanins byVitisCells in Suspension Culture

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Abstract
A large amount of anthocyanin was accumulated in the callus tissues of Vitis hybrids without light irradiation. Culture conditions for the production of anthocyanins by Vitis cells in suspension cultures were investigated. High sucrose and low phosphate concentrations brought about a marked increase of anthocyanin formation, while high concentrations of nitrate, phosphate, and 2, 4-D repressed the pigment formation. The effects of these nutrients depended on the concentrations of coexistent ones. Regulation of the aeration rate was important for anthocyanin formation in submerged aerated cultures and light irradiation enhanced anthocyanin formation in cultured cells.