Immunolocalization of Dinitrogenase Reductase Produced by Klebsiella pneumoniae in Association with Zea mays L
- 1 February 2000
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 66 (2) , 783-7
- https://doi.org/10.1128/aem.66.2.783-787.2000
Abstract
The endophytic lifestyle of Klebsiella pneumoniae is described, including the production of dinitrogenase reductase by bacteria residing in maize root tissue. The green fluorescent protein (GFP) was used to detect the colonization of maize by K. pneumoniae strains 2028 and 342. These strains were found to reside in intercortical layers of the stem and within the region of maturation in the root. The production of dinitrogenase reductase by GFP-tagged bacteria was visualized using immunolocalization. This activity was only apparent when bacteria were supplied with an exogenous carbon source. The results suggest that maize provides a suitable habitat for K. pneumoniae and that this species is capable of producing nitrogenase under the appropriate plant cultivation conditions.Keywords
This publication has 27 references indexed in Scilit:
- Endophytic Expression of nif Genes of Azoarcus sp. Strain BH72 in Rice RootsMolecular Plant-Microbe Interactions®, 1999
- Use of Green Fluorescent Protein to Detect Expression of nif Genes of Azoarcus sp. BH72, a Grass-Associated Diazotroph, on Rice RootsMolecular Plant-Microbe Interactions®, 1998
- Gapped BLAST and PSI-BLAST: a new generation of protein database search programsNucleic Acids Research, 1997
- Herbaspirillum, an endophytic diazotroph colonizing vascular tissue 3Sorghum bicolor L. MoenchJournal of Experimental Botany, 1997
- Flow cytometric and microscopic analysis of GFP-tagged Pseudomonas fluorescens bacteriaFEMS Microbiology Ecology, 1997
- GREEN FLUORESCENT PROTEINPhotochemistry and Photobiology, 1995
- Infection of sugar cane by the nitrogen-fixing bacteriumAcetobacter diazotrophicusJournal of Experimental Botany, 1994
- Comparative study of the response ofAzotobacter vinelandii andAcetobacter diazotrophicus to changes in pHProtoplasma, 1994
- Use oflacZFusions to Study the Expression ofnifGenes ofAzospirillum brasilensein Association with PlantsMolecular Plant-Microbe Interactions®, 1994
- Spatial-Temporal Colonization Patterns ofAzospirillum brasilenseon the Wheat Root Surface and Expression of the BacterialnifHGene during AssociationMolecular Plant-Microbe Interactions®, 1993