Simplified method for producingParacoccidioidesbrasiliensisexoantigens for use in immunodiffusion tests
Open Access
- 1 January 2003
- journal article
- Published by Oxford University Press (OUP) in Medical Mycology
- Vol. 41 (6) , 539-542
- https://doi.org/10.1080/13693780310001615358
Abstract
A simplified method to produce Paracoccidioidesbrasiliensis exoantigens for immunodiffusion testing is proposed. It uses technology accessible for small laboratories with few resources in Latin America, where paracoccidioidomycosis is endemic. This procedure may replace the more complex procedure, originally proposed by Camargo etal. in 1988, that is currently commonly used. It is based on the production of exoantigen by P.brasiliensis isolate B339, a good secretor of the characteristic 43 000-Da glycoprotein gp43.Keywords
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