Studies on the Na+-K+-ATPase in myocardial infarction.

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Abstract
Changes in the cardiac sarcolemma in myocardial infarction were studied by both determination of Na+-K+-ATPase activity and SDS [sodium dodecylsulfate] gel electrophoretic analysis of sarcolemmal proteins in the canine heart. Ninety min after coronary ligation, Na+-K+-ATPase activity in ischemic myocardium was decreased significantly to approximately 36% of that of non-ischemic myocardium and it remained at the lower level for 28 days. By SDS gel electrophoresis, reduction of the protein band with MW of 111,000, which is suggestive of the main component of ATPase, was observed simultaneously with the reduction of Na+-K+-ATPase activity. Ischemia for 90 min apparently produces substructural changes in the sarcolemma indicating irreversible myocardial changes.