THE REDUCTION OF METHYLENE BLUE BY HYDROGENASE

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Abstract
Sonicates of Micrococcus lactilyticus catalyse the hydrogenation of a wide variety of chemical compounds. Hydrogenation of some of these compounds is reversible, i.e., H2 is evolved from reduced methyl viologen, reduced benzyl viologen and hypoxanthine in a N2 atmosphere. Preparations of "soluble hydrogenase" obtained by fractionation of sonicates of M. lactilyticus exhibit high specific activity when assayed with benzyl viologen and certain other dyes, but show little or no H-uptake with methylene blue. Evidence was obtained that a component, in addition to hydrogenase, is required for reduction of methylene blue. Methylene blue activity is restored to preparations of "soluble hydrogenase" from M. lactilyticus by addition of various heat-labile materials.

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