Vitamin B12 transport in Escherichia coli: energy coupling between membranes
- 1 December 1990
- journal article
- review article
- Published by Wiley in Molecular Microbiology
- Vol. 4 (12) , 2027-2033
- https://doi.org/10.1111/j.1365-2958.1990.tb00562.x
Abstract
Cells of Escherichia coli possess high-affinity active transport systems of vitamin B12 and iron-siderophore complexes. Specific outer-membrane proteins carry out the energy-dependent transport across the outer membrane, in conjunction with the TonB coupling protein. Mutagenesis experiments have identified a conserved region near the amino-terminus of the outer-membrane transporters that is necessary for energy-coupled transport. The ability of extragenic suppressor mutations in tonB to correct the transport defect indicates that TonB couples the proton-motive force to the outer-membrane proteins by direct contact.Keywords
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