THE RELATIONSHIP BETWEEN GROWTH AND MUTATION IN PSEUDOMONAS FLUORESCENS
- 1 August 1949
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 58 (2) , 171-180
- https://doi.org/10.1128/jb.58.2.171-180.1949
Abstract
A mutant of P. fluorescens was discovered which uses itaconate (methylene succinate) as the sole source of C for growth, the parent being unable to do so. By means of a simplified tube fluctuation test the random origin of the mutation was established, and a high positive correlation between growth of the wild type and the mutation to itaconate utilization was demonstrated. Furthermore it was shown that P. fluorescens can grow for many generations to a population density below the visible level in a medium devoid of any deliberately added utilizable carbon source. A new simplified method was used for the detn. of the mutation rate based upon a modification of the tube fluctuation test, employing an equation derived by Luria and Delbriick. The mutation rate so calculated, based upon the averaging of 3, is 1.9 x 10-8. This method may be used in detn. of mutation rates involving mutations which are expressed phenotypically by "added" biochemical properties.This publication has 5 references indexed in Scilit:
- The Oxidation of Aromatic Compounds by Fluorescent PseudomonadsJournal of Bacteriology, 1948
- TRAINING OR MUTATION OF BACTERIA1948
- The Relative Errors of Bacteriological Plate Counting MethodsJournal of Bacteriology, 1947
- MUTATIONS IN ESCHERICHIA COLI INDUCED BY CHEMICAL AGENTSCold Spring Harbor Symposia on Quantitative Biology, 1947
- MUTATIONS OF BACTERIA FROM VIRUS SENSITIVITY TO VIRUS RESISTANCEGenetics, 1943