Direct PCR of whole blood and hair shafts by microwave treatment.

  • 1 October 1994
    • Vol. 17  (4) , 726, 728
Abstract
We report a simple and rapid method for DNA preparation suitable for PCR by microwave irradiation. When mouse whole blood and hair shafts were directly irradiated and subjected to PCR, a unique gene such as the retinoblastoma susceptibility gene was reproducibly amplified. This method was useful for screening a transgene sequence in transgenic mice.

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