Microdetermination of plasma ATP and creatine phosphate concentrations with a luminescence biometer.
- 1 December 1977
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 23 (12) , 2254-2257
- https://doi.org/10.1093/clinchem/23.12.2254
Abstract
We optimized conditions for determination of adenosine-5'-triphosphate (ATP) and creatine phosphate from plasma extracted with ethanol/water (96/4 by vol). The procedures utilize the firefly luciferin/luciferase reaction, the bioluminescence being measured with a Du Pont Biometer. ATP is quantitated directly and creatine phosphate is quantitated by reaction with creatine kinase and ADP, after plasma ATP is removed by incubation with the enzyme apyrase. The method is applied to plasma from humans, rabbits, and rats, and possible clinical applications are discussed.This publication has 11 references indexed in Scilit:
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