Biochemical characterization of the fidelity of poliovirus RNA-dependent RNA polymerase
Open Access
- 24 May 2007
- journal article
- Published by Springer Nature in Virology Journal
- Vol. 4 (1) , 44
- https://doi.org/10.1186/1743-422x-4-44
Abstract
Putative high mutation rates of RNA viruses are believed to mediate undesirable phenomena, such as emergence of drug resistance. However, very little is known about biochemical fidelity rates for viral RNA-dependent RNA polymerases. Using a recently developed in vitro polymerase assay for poliovirus polymerase 3Dpol [Arnold and Cameron (2000) JBC 275:5329], we measured fidelity for each possible mismatch. Polymerase fidelity, in contrast to sequence error rate, is biochemically defined as kpol/Kd of {(correct plus incorrect) divided by incorrect} incorporations, such that a larger value connotes higher fidelity.Keywords
This publication has 40 references indexed in Scilit:
- Poliovirus RNA-Dependent RNA Polymerase (3Dpol): Pre-Steady-State Kinetic Analysis of Ribonucleotide Incorporation in the Presence of Mn2+Biochemistry, 2004
- Poliovirus RNA-Dependent RNA Polymerase (3Dpol): Pre-Steady-State Kinetic Analysis of Ribonucleotide Incorporation in the Presence of Mg2+Biochemistry, 2004
- Poliovirus RNA-Dependent RNA Polymerase (3Dpol): Kinetic, Thermodynamic, and Structural Analysis of Ribonucleotide SelectionBiochemistry, 2004
- Lethal mutagens: broad-spectrum antivirals with limited potential for development of resistance?Drug Resistance Updates, 2004
- Serial Recombination during Circulation of Type 1 Wild-Vaccine Recombinant Polioviruses in ChinaJournal of Virology, 2003
- Viral RNA Mutations Are Region Specific and Increased by Ribavirin in a Full-Length Hepatitis C Virus Replication SystemJournal of Virology, 2002
- Structure-Function Relationships of the RNA-dependent RNA Polymerase from Poliovirus (3Dpol)Journal of Biological Chemistry, 2002
- Efficient Virus Extinction by Combinations of a Mutagen and Antiviral InhibitorsJournal of Virology, 2001
- Ribavirin Induces Error-Prone Replication of GB Virus B in Primary Tamarin HepatocytesJournal of Virology, 2001
- Poliovirus RNA-dependent RNA Polymerase (3Dpol) Is Sufficient for Template Switchingin VitroJournal of Biological Chemistry, 1999