APOBEC3G Incorporation into Human Immunodeficiency Virus Type 1 Particles
Open Access
- 1 November 2004
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 78 (21) , 12058-12061
- https://doi.org/10.1128/jvi.78.21.12058-12061.2004
Abstract
APOBEC3G is promiscuous with respect to its antiretroviral effect, requiring that it be packaged into diverse retrovirus particles. Here, we show that most virally encoded human immunodeficiency virus type 1 particle components are dispensable for APOPEC3G incorporation. However, replacement of the nucleocapsid (NC) Gag domain with a leucine zipper abolished APOBEC3G incorporation. Moreover, coprecipitation analysis showed that APOBEC3G-Gag interaction requires NC and nonspecific RNA. These observations suggest that APOBEC3G exploits an essential property of retroviruses, namely, RNA packaging, to infiltrate particles. Because it is, therefore, difficult to evolve specific sequences that confer escape from APOBEC3G, these findings may explain why lentiviruses evolved an activity that induces its destruction.Keywords
This publication has 26 references indexed in Scilit:
- Capsid-Dependent and -Independent Postentry Restriction of Primate Lentivirus Tropism in Rodent CellsJournal of Virology, 2004
- Induction of APOBEC3G Ubiquitination and Degradation by an HIV-1 Vif-Cul5-SCF ComplexScience, 2003
- The Human Immunodeficiency Virus Type 1 Vif Protein Reduces Intracellular Expression and Inhibits Packaging of APOBEC3G (CEM15), a Cellular Inhibitor of Virus InfectivityJournal of Virology, 2003
- Species-Specific Exclusion of APOBEC3G from HIV-1 Virions by VifCell, 2003
- DNA Deamination Mediates Innate Immunity to Retroviral InfectionCell, 2003
- The cytidine deaminase CEM15 induces hypermutation in newly synthesized HIV-1 DNANature, 2003
- Broad antiretroviral defence by human APOBEC3G through lethal editing of nascent reverse transcriptsNature, 2003
- Isolation of a human gene that inhibits HIV-1 infection and is suppressed by the viral Vif proteinNature, 2002
- High-Level Transduction and Gene Expression in Hematopoietic Repopulating Cells Using a Human Imunodeficiency Virus Type 1-Based Lentiviral Vector Containing an Internal Spleen Focus Forming Virus PromoterHuman Gene Therapy, 2002
- An Anthropoid-Specific Locus of Orphan C to U RNA-Editing Enzymes on Chromosome 22Genomics, 2002