Application of Nonlinear Regression Analysis to the use of Enzymes in The Determination of Substrate Concentrations

1 January 1982

journal article
research article
Published by Taylor & Francis in Instrumentation Science & Technology

Vol. 12 (2) , 125-134
https://doi.org/10.1080/10739148208543552

Abstract

A modified nonlinear regression analysis program (originally reported by R.G. Duggleby (1981) Anal. Biochem. 110, 9–18) in BASIC for microcomputers and a modified spectrophotometer are described. Their application in the routine determination of concentrations of certain substances by the use of specific enzymes is detailed. Results are given that show the utility of the system for glucose determination by the hexokinase reaction, and its lack of utility for lactate determination using lactate dehydrogenase.

Keywords

This publication has 4 references indexed in Scilit:

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A comparison of two methods for fitting the integrated Michaelis–Menten equation
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Statistical Estimations in Enzyme Kinetics
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