A calcium‐dependent protein kinase is present in tetrahymena

1 September 1994

journal article
research article
Published by Wiley in Cell Biochemistry and Function

Vol. 12 (3) , 221-226
https://doi.org/10.1002/cbf.290120311

Abstract

A Ca²⁺‐dependent protein kinase of Tetrahymena thermophila has been partially purified and characterized. The molecular mass of the enzyme is less than that of similar enzymes (for example protein kinase C), being about 55 kDa. After purification and in the presence of Ca²⁺ the enzyme activity increased. The promoter of protein kinase C (PKC) activity, phorbol myristate acetate (PMA), increased the activity while the protein kinase inhibitor H‐7 decreased the activity of the enzyme. The experiments demonstrate the presence, activity and similarity to vertebrate enzymes of a protein kinase at a low level of phylogeny.

Keywords

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