Substances Inhibitory to Insect Feeding with Insecticidal Properties from Fungi1

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Abstract
Two compounds, designated respectively 379-X and 370-Y, which first markedly inhibited the feeding activity of adults and larvae of the Mexican bean beetle (Epilachna varivestis Mulsant), and subsequently caused their death, were isolated from culture filtrates of a soil fungus identified as Myrothecium roridum Tode. The compounds were active when applied to bean leaves on which the beetles fed and when applied topically to the insects. A tentative molecular formula for compound 379-X was determined as C29H49O9. Other characteristics were: M. W. (Rast) 492; n.v. absorption maximum, 261 mu; m.p. 190 C; [α]D25 = +78° (4.8% in dioxane). It was soluble in most organic solvents and to the extent of 10-5M. in water. Compound 379-Y was tentatively assigned the molecular formula C49H64O16. Other characteristics: M.W. (Rast) 903; u.v. max., 259 mu; decomposed at 295° C.; [α]D25 = +200.5° (6.3% in dioxane), +226.5° (0.4% in benzene); soluble in most organic solvents, and to the extent of 10-6M. in water.

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