Epidemiological classification of infectious bronchitis virus isolated in Korea between 1986 and 1997
- 1 August 1998
- journal article
- Published by Taylor & Francis in Avian Pathology
- Vol. 27 (4) , 409-416
- https://doi.org/10.1080/03079459808419360
Abstract
Forty Korean isolates and four reference strains of infectious bronchitis virus (IBV) were classified by reverse transcriptase-polymerase chain reaction and restriction fragment length polymorphism (RFLP) analysis. Each Korean isolate was isolated from different types of commercial chicken flocks between 1986 and 1997. RFLP patterns of an amplified DNA fragment (1722 bp) containing the S1 gene of IBV digested by restriction enzyme HaeIII showed that the 40 Korean isolates were classified into five genotypes, I to V. Six of them belonged to genotype I which had the same HaeIII and XcmI cleavage patterns with Massachusetts type (H120 and M41) but the other four genotypes had a different HaeIII cleavage pattern from the four reference IBV strains used in this study. Genotype III seemed to be the major type as 29 of the 40 isolates belonged to this type which was consistently found in the chicken flocks since 1990. On the other hand, genotypes II, IV and V were found in the field only in 1986, 1995 and 1995, respectively. Five isolates selected from each of the five genotypes were inoculated into 1-day-old specific-pathogen-free chicks to evaluate their pathogenicity. Genotype III induced 50% mortality as well as severe renal urate deposition on the kidneys but the other four genotypes only showed respiratory distress at 1 to 2 days after inoculation. Live H120 vaccine protected chicks against challenge with isolates selected from genotype I, but not genotypes IV to V. A live KM91p120 strain selected from major genotype III did protect chicks against challenge with isolates from genotype III, in addition to other genotypes, including two recent isolates of genotypes IV and V.Keywords
This publication has 7 references indexed in Scilit:
- Challenge experiments to evaluate cross‐protection induced at the trachea and kidney level by vaccine strains and Belgian nephropathogenic isolates of avian infectious bronchitis virusAvian Pathology, 1993
- Evidence of Natural Recombination within the S1 Gens of Infectious Bronchitis VirusVirology, 1993
- Differentiation of Infectious Bronchitis Virus Serotypes Using Polymerase Chain Reaction and Restriction Fragment Length Polymorphism AnalysisPublished by JSTOR ,1993
- Location of antigenic sites defined by neutralizing monoclonal antibodies on the S1 avian infectious bronchitis virus glycopolypeptideJournal of General Virology, 1992
- Location of the amino acid differences in the S1 spike glycoprotein subunit of closely related serotypes of infectious bronchitis virusAvian Pathology, 1992
- Molecular Epidemiology of Infectious Bronchitis Virus in The NetherlandsJournal of General Virology, 1987