Adaptation of the primer extension preamplification (PEP) reaction for preimplantation diagnosis: single blastomere analysis using short PEP protocols
- 1 January 1996
- journal article
- research article
- Published by Oxford University Press (OUP) in Molecular Human Reproduction
- Vol. 2 (3) , 209-212
- https://doi.org/10.1093/molehr/2.3.209
Abstract
Primer extension preamplification (PEP) was first described as a method for whole genome amplification, starting from a single cell, originally a spermatozoon, in order to perform genetic recombination studies. Its usefulness for preimplantation diagnosis was shown soon after; the only drawback was the length of the procedure (> 14 h). We have developed a shorter PEP protocol for single human blastomeres, enabling us to examine several genetic loci of interest in human genetic diseases with a good amplification efficiency.Keywords
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