ELECTRON STAINING WITH URANYL ACETATE POSSIBLE ROLE OF FREE AMINO GROUPS

1 March 1971

journal article
Published by SAGE Publications in Journal of Histochemistry & Cytochemistry

Vol. 19 (3) , 161-168
https://doi.org/10.1177/19.3.161

Abstract

Uranyl acetate (UA) is known to have affinity for nucleic acids and –COOH groups. Experiments were performed to test the possible affinity of UA for free amino groups. For this purpose, –NH₂ blocking and deamination techniques (with dinitrofluorobenzene, nitrous acid and Chloramine T) were applied to form-aldehyde-fixed pancreas samples, after which the stainability of zymogen granules by UA was strongly reduced. It is concluded that free amino groups may also participate in the binding of UA in biologic specimens.

Keywords

AFFINITY
URANYL ACETATE
DINITROFLUOROBENZENE
FREE AMINO GROUPS

This publication has 0 references indexed in Scilit: