Neutral amino acid transport during the cell cycle of cultured human lymphocytes
- 1 April 1981
- journal article
- research article
- Published by Wiley in Journal of Cellular Physiology
- Vol. 107 (1) , 69-74
- https://doi.org/10.1002/jcp.1041070109
Abstract
Neutral amino acid (isoleucine, leucine, and valine) transport was monitored in synchronous populations of WIL‐2 cells, a diploid human lymphoblastoid B cell line. Cells were synchronized by either the double thymidine block technique or by density‐dependent arrest in G1. Cells synchronized by these methods showed up to a 66% decrease in amino acid transport upon progressing from the early S phase through the G2 and M phases with a concomitant increase in transport during the G1 phase. The lowest activities of amino acid transport in synchronized cells were observed during the G2 and M phases.Keywords
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