IMMUNE-RESPONSE TO UNCOUPLED PEPTIDES OF FOOT-AND-MOUTH-DISEASE VIRUS
- 1 May 1987
- journal article
- research article
- Vol. 61 (1) , 1-6
Abstract
Uncoupled synthetic peptide representing the sequence of amino acids 141-160 of foot-and-mouth disease virus (FMDV) protein VP1 induced a virus-neutralizing antibody response in guinea-pigs. This response required incomplete Freund''s adjuvant (IFA) for the primary inoculation and was dependent on the presence of an added cysteine residue with an unblocked sulphydryl group at the carboxy-terminus. Secondary immunization could be carried out in the absence of adjuvant. A study of the relative activities of nested sets of uncoupled peptides from 150-160 to 135-160 and 141-160 to 141-155 indicated that amino acids 146-156 were critical for the induction of virus-neutralizing antibodies and that extension to 137-160 further improved this response. Results of in vitro proliferation studies demonstrated that the carboxy-terminal residues on this peptide may form a T-cell epitope. The significance of these observations in the broader context of synthetic peptide vaccines is discussed.This publication has 16 references indexed in Scilit:
- Immunological Priming with Synthetic Peptides of Foot-and-Mouth Disease VirusJournal of General Virology, 1985
- T-cell antigenic sites tend to be amphipathic structures.Proceedings of the National Academy of Sciences, 1985
- Radioimmunoassay for detection of VP1 specific neutralizing antibodies of foot and mouth disease virusJournal of Virological Methods, 1985
- AFFINITY OF ANTIBODY RESPONSES IN MAN TO HEPATITIS B VACCINE DETERMINED WITH SYNTHETIC PEPTIDESThe Lancet, 1984
- Demonstration of Neutralizing and Non-neutralizing Epitopes on the Trypsin-sensitive Site of Foot-and-Mouth Disease VirusJournal of General Virology, 1984
- Antibody response in pig nasal fluid and serum following foot-and-mouth disease infection or vaccinationEpidemiology and Infection, 1983
- Protection against foot-and-mouth disease by immunization with a chemically synthesized peptide predicted from the viral nucleotide sequenceNature, 1982
- Antibody to hepatitis B surface antigen after a single inoculation of uncoupled synthetic HBsAg peptidesNature, 1982
- A simple reliable system for studying antigen-specific murine T cell proliferationJournal of Immunological Methods, 1979
- Purification of Radioactive Foot-and-Mouth Disease VirusNature, 1963