Adenylate Cyclase from Brevibacterium liquefaciens . III. In Situ Regulation of Adenylate Cyclase by Pyruvate
- 1 November 1974
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 71 (11) , 4598-4601
- https://doi.org/10.1073/pnas.71.11.4598
Abstract
In the presence of DL-alanine intracellular cyclic AMP in nonproliferating cells of Brevibacterium liquefaciens increased rapidly to the maximum level of approximately 180 muM, and extracellular cyclic AMP increased to 100 muM within 4 hr at 25 degrees . Adenylate cyclase (EC 4.6.1.1) induction was not observed during this incubation. The concentration of pyruvate in the total culture increased concomitantly with that of cyclic AMP and reached approximately 20 mM after 4 hr of incubation. Since the activity of cyclic nucleotide phosphodiesterase is extremely low in this bacterium, the accumulation of cyclic AMP with DL-alanine appeared to be due to the activation of adenylate cyclase by pyruvate. D-alanine was more effective than L-alanine in producing pyruvate, and a high activity of D-alanine oxidation was detected in the cell lysate of B. liquefaciens.Thus, adenylate cyclase in this bacterium appeared to be regulated in vivo by pyruvate which was formed, in this case, predominantly from D-alanine through the action of D-aminoacid oxidase (EC 1.4.3.3). Pyruvate, added extracellularly, also caused a rapid accumulation of intracellular cyclic AMP. Glucose did not change the level of cyclic AMP significantly. It also did not affect the intracellular accumulation of cyclic AMP with DL-alanine.Keywords
This publication has 7 references indexed in Scilit:
- Adenylate cyclase from Brevibacterium liquefaciens. I. Purification, crystallization, and some properties.1974
- Glucose and the Metabolism of Adenosine 3′:5′-Cyclic Monophosphate in Escherichia coliProceedings of the National Academy of Sciences, 1971
- A Protein Binding Assay for Adenosine 3′:5′-Cyclic MonophosphateProceedings of the National Academy of Sciences, 1970
- Use of the liquid scintillation spectrometer for determining adenosine triphosphate by the luciferase enzymeAnalytical Biochemistry, 1969
- Adenyl cyclase of Brevibacterium liquefaciensBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1967
- ADENOSINE 3',5'-PHOSPHATE IN ESCHERICHIA COLI1965
- Excretion of adenosine-3′,5′-phosphate in the culture broth of Brevibacterium liquefaciensArchives of Biochemistry and Biophysics, 1963