Immunofluorescence on resin-embedded material

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Abstract
Some antigenic determinants can be preserved in tissues after plastic embedding. In the present study [human and rabbit] liver tissue was fixed with glutaraldehyde or paraformaldehyde, dehydrated with ethanol and toluene and embedded in araldite which was then polymerized at 37.degree. C. Immunofluorescence was performed on semi-thin sections etched with hydrogen peroxide. This procedure allows correlation with light microscopy (on the same stained semi-thin section) and with EM (on adjacent ultra-thin section). Good results were obtained with anti-nuclear, anti-mitochondrial, anti-microsomal and anti-.alpha.1-antitrypsin sera.

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