α‐Synuclein implicated in Parkinson's disease is present in extracellular biological fluids, including human plasma
Top Cited Papers
- 15 August 2003
- journal article
- Published by Wiley in The FASEB Journal
- Vol. 17 (13) , 1-16
- https://doi.org/10.1096/fj.03-0098fje
Abstract
SPECIFIC AIMSWe investigated whether neuronal cells in culture normally secrete α-synuclein protein (α-syn) into their surrounding media. We also looked for the presence of α-syn in human cerebrospinal fluid (CSF) and blood plasma. The methods used were immunocapture with anti-α-syn antibodies coupled to magnetic beads, followed by detection on Western blots using five different antibodies to α-syn.PRINCIPAL FINDING1. Neuronal cells normally secrete α-synTo determine whether cells secrete α-syn during normal metabolism, we used human neuroblastoma M17 cells stably transfected with cDNA encoding the wild-type form of α-syn (M17-wt), untransfected M17 cells, or cells transfected with vector alone (M17-vec). Serum-free OPTI-MEM medium conditioned for 6–48 h with each cell type was immunoprecipitated using magnetic dynabeads coupled to 211 mouse monoclonal antibody which recognizes amino acid residues 121-125 of α-syn. Bound proteins were eluted from the beads, after washing, and separated on SDS-gels before ...Keywords
This publication has 42 references indexed in Scilit:
- Review: Formation and Properties of Amyloid-like Fibrils Derived from α-Synuclein and Related ProteinsJournal of Structural Biology, 2000
- Acceleration of oligomerization, not fibrillization, is a shared property of both α-synuclein mutations linked to early-onset Parkinson's disease: Implications for pathogenesis and therapyProceedings of the National Academy of Sciences, 2000
- Effects of the mutations Ala30 to Pro and Ala53 to Thr on the physical and morphological properties of α‐synuclein protein implicated in Parkinson's diseaseFEBS Letters, 1998
- Multiple-system atrophy: a new α-synuclein disease?The Lancet, 1998
- Filamentous α-synuclein inclusions link multiple system atrophy with Parkinson's disease and dementia with Lewy bodiesNeuroscience Letters, 1998
- AlaSOPro mutation in the gene encoding α-synuclein in Parkinson's diseaseNature Genetics, 1998
- α-Synuclein in Lewy bodiesNature, 1997
- Mutation in the α-Synuclein Gene Identified in Families with Parkinson's DiseaseScience, 1997
- Identification of two distinct synucleins from human brainFEBS Letters, 1994
- Molecular cloning of cDNA encoding an unrecognized component of amyloid in Alzheimer disease.Proceedings of the National Academy of Sciences, 1993