Isolation and Characterization of Human Skeletal Muscle Creatine Kinase
- 1 January 1973
- journal article
- research article
- Published by Taylor & Francis in Preparative Biochemistry
- Vol. 3 (3) , 199-208
- https://doi.org/10.1080/00327487308061506
Abstract
Human skeletal muscle creatine kinase was purified by isoelectric focusing with a yield of greater than 60%. Two enzymic proteins, differing in specific activity, were obtained, and each final product produced only a single protein band when examined by electrophoretic methods. The proteins were composed of two subunits of about 41, 000 daltons each, and the amino acid compositions were similar.Keywords
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