Quantitation of Clostridium perfringens Type A Enterotoxin by Electroimmunodiffusion

Open Access

1 January 1972

journal article
research article
Published by American Society for Microbiology in Applied Microbiology

Vol. 24 (5) , 801-804
https://doi.org/10.1128/aem.24.5.801-804.1972

Abstract

Conditions for quantitation of Clostridium perfringens type A enterotoxin by electroimmunodiffusion are described. As little as 0.01 μg of enterotoxin could be detected. Electroimmunodiffusion was more sensitive than either single gel diffusion or quantitation based on erythemal activity of the toxin in guinea pig skin.

Keywords

CLOSTRIDIUM PERFRINGENS
GUINEA PIG
ENTEROTOXIN
QUANTITATION BASED
PERFRINGENS TYPE
GEL DIFFUSION
ERYTHEMAL ACTIVITY
SINGLE GEL