Quantitative Bestimmung von RNA- und DNA-Gehalten in Säugetierorganen

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Abstract
The determination of the tissue concentration of RNA and DNA from the sum of the mononucleo-tides or bases (DNA) after hydrolysis is an exact but involved procedure. Separation of the nucleic acid derivatives by ion exchange chromato-graphy, standardization of the quantitative determination, and the treatment of the tissue are described. The amounts of nucleic acids determined in the organs of various species [mouse, rat, rabbit liver, rat, rabbit kidney, cat pancreas, dog testicle, rabbit brain and heart] were compared with the values in the literature. The determination of RNA by UV -measurement after alkaline hydrolysis, modifications of this method sources of error, and deviations from the chromatographic values are discussed.