Magnetic molecularly imprinted polymer beads for drug radioligand binding assay
- 1 January 1998
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in The Analyst
- Vol. 123 (7) , 1611-1616
- https://doi.org/10.1039/a801903g
Abstract
Molecularly imprinted polymer–magnetic iron oxide composite materials which exhibit recognition properties and can be withdrawn from solution by application of a magnetic field were prepared for the first time. Magnetic iron oxide was incorporated using a suspension polymerisation methodology with a perfluorocarbon liquid as the dispersing phase for the preparation of methacrylic acid-1,1,1-trimethylolpropane trimethacrylate copolymer beads molecularly imprinted with the β-blocker (S)-propranolol. The resulting superparamagnetic imprinted polymer beads were capable of binding [3H]-(S)-propranolol more strongly than a non-imprinted, otherwise identical, polymer. In a competitive radioligand binding assay using a magnet to separate polymer from solution, (R)-propranolol and (R,S)-metoprolol exhibited cross-reactivities of 19 and 0.7%, respectively, compared with (S)-propranolol.Keywords
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