Molecular cloning and sequencing of mRNAs coding for minor adult globin polypeptides of Xenopus laevis
Open Access
- 11 March 1983
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 11 (5) , 1543-1553
- https://doi.org/10.1093/nar/11.5.1543
Abstract
Globin mRNA was isolated from immature red blood cells of an adult Xenopus laevis female. mRNA/cDNA hybrids were integrated in the Pst I cleavage site of pBR 322 by G/C tailing, and cloned in Escherichia coli strain HB 101. By restriction site analysis as well as hybridization behaviour we identified two clones coding for minor adult α and β globin chains. Nucleotide sequence analysis and derived amino acid sequences are presented.Keywords
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