SEQUENCE DEPENDENT DEAMIDATION RATES FOR MODEL PEPTIDES OF HEN EGG‐WHITE LYSOZYME
- 1 July 1973
- journal article
- Published by Wiley in International Journal of Peptide and Protein Research
- Vol. 5 (4) , 275-278
- https://doi.org/10.1111/j.1399-3011.1973.tb03461.x
Abstract
The rates of deamidation in pH 7.5, 0.15 M, 37°C potassium phosphate buffer of eleven pentapeptide models of the sequences found near asparaginyl and glutaminyl residues in egg white lysozyme were measured. These rates and rate estimates from previous measurements were used to calculate a sequence‐determined half‐life for non‐deamidated egg white lysozyme. Deamidation of the peptides during synthesis and purification was also measured. The implications of these measurements for biological deamidation and deamidation during peptide synthesis are discussed.This publication has 13 references indexed in Scilit:
- Lysozyme turnover in the ratJournal of Clinical Investigation, 1971
- Controlled Deamidation of Peptides and Proteins: An Experimental Hazard and a Possible Biological TimerProceedings of the National Academy of Sciences, 1970
- The primary structure of duck egg-white lysozyme IIBiochimica et Biophysica Acta (BBA) - Protein Structure, 1970
- Use of Anhydrous Hydrogen Fluoride in Peptide Synthesis. I. Behavior of Various Protective Groups in Anhydrous Hydrogen FluorideBulletin of the Chemical Society of Japan, 1967
- Use of Ilydrogen Fluoride in Merrifield Solid-Phase Peptide SynthesisJournal of the American Chemical Society, 1967
- The Synthesis of Bovine Insulin by the Solid Phase Method1Journal of the American Chemical Society, 1966
- Synthesis of Angiotensins by the Solid-Phase Method*Biochemistry, 1965
- Solid Phase Peptide Synthesis. II. The Synthesis of BradykininJournal of the American Chemical Society, 1964
- Homnogeneity and the Electrophoretic Behavior of Some Proteins. H. Reversible Spreading and Steady-State Boundary CriteriaThe Journal of Physical Chemistry, 1948
- Homogencity and the Electrophoretic Behavior of Some ProteinsThe Journal of Physical Chemistry, 1948