DETERMINATION OF FLUMEQUINE IN MILK BY THIN-LAYER CHROMATOGRAPHY-BIOAUTOGRAPHY

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Abstract
Fluoroquinolones are synthetic antibiotics widely used in human and animal medical treatment. Flumequine, belonging to this group, is often used in medical treatment and prevention in livestock. It is also added to feed or water to obtain better weight gain. Many physicochemical techniques have been reported for the assay of fluoroquinolones. Thin-layer chromatography (TLC) is a method of choice for analysis because it is cheap and simple, provides high sample throughput, and requires limited sample pre-treatment. Thin-layer chromatography-bioautography (TLC-B) is the screening technique, which combines TLC with microbiological detection resulting in enhanced sensitivity. In this paper, a simple TLC-B method for determination of flumequine in milk is described. The sample pre-treatment is performed directly on the plate. Microbiological detection directly on the plate allows for enhanced sensitivity of the method.