Error analysis in flow microfluorometry
- 1 March 1982
- journal article
- Published by Springer Nature in Cell Biochemistry and Biophysics
- Vol. 4 (1) , 1-14
- https://doi.org/10.1007/bf02788551
Abstract
Sources of error in a typical algorithm for the analysis of single flow-microfluorometric histograms are identified. A new statistical model for such data is presented, by means of which the error sources are quantitatively investigated. These theoretical investigations lead to three practical observations: A more detailed characterization of the fluorescence dispersion process is needed for a more refined algorithm. Levels of dispersion typically experienced are such that from a single histogram the distribution of cells within S-phase cannot be finely resolved; but the crude distribution of cells among the three phases G1, S, and G2-M may be accurately estimated. If currently typical levels of dispersion can be halved, then the S-phase distribution can be finely resolved.Keywords
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