Production and characterization of ligninolytic enzymes of Bjerkandera adusta grown on wood meal/wheat bran culture and production of these enzymes using a rotary-solid fermenter

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Abstract
Manganese peroxidase (MnP) and lignin peroxidase (LIP) were produced by growing a white-rot fungus Bjerkandera adusta statically, on a wood meal/wheat bran culture in flasks. MnP and LiP reached their maximum activity after 6 and 19 days of inoculation, respectively. Both MnP and LiP are thought to be important enzymes in lignin biodegradation by B. adusta. Ion exchange chromatography showed that B. adusta produced a single LiP and a single MnP enzyme in wood meal/wheat bran culture. These enzymes were separated and characterized. The molecular weight of MnP was 46,500 with a p/of 3.9. The molecular weight of LiP was estimated to be 47,000 with a p/of 3.5. Spectral analysis demonstrated that both enzymes are heme proteins. Production of these enzymes was also achieved using a rotarysolid culture fermenter. MnP, LiP and veratryl alcohol oxidase were produced by B. adusta in the fermenter.

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