Ester Production by Pseudomonas fragi IV. Demonstration of Esterase Activity 1

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Abstract
Assay of the esterase activity of sonically treated cell-free extracts, whole cell suspensions, and supernatant fluid of Pseudomonas fragi cultures with a differential respirometer revealed that the esterases were intracellular. Polyacrylamide-gel electrophoresis demonstrated six bands of esterase activity, which revealed substrate specificity differences. Band 1 exhibited slow mobility, bands 2, 3, and 4 moderate mobility, and bands 5 and 6 rapid mobility. Six bands were active with α-naphthyl acetate, four bands with α-naphthyl propionate, and 5 bands with αnaphthyl butyrate. These esterases appeared to be more active with aromatic esters than with aliphatic esters.

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