Localization of reactive tyrosine residues of baker's yeast transketolase

Abstract
Baker's yeast transketolase inactivated by tetranitromethane was digested with Staphylococcus aureus V8 protease. Four peptides absorbing at 360 nm were isolated by reverse-phase HPLC and sequenced. The modified tyrosines were identified as Tyr-184, Tyr-210 and Tyr-370