Abstract
The development of a chicken-embryo-lethal strain of duck hepatitis virus is described. The strain was obtained through serial passage of the virus in chicken embryos, and a total of 80 passages were carried out. Embryo mortality was consistently 100% in 2 to 5 days from the 63rd passage on. Possible use of this strain for conducting virus neutralizing tests in chicken embryos and as a vaccine strain for immunizing ducklings is discussed.

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