Quantitation of phosphatidyl N‐methyl‐ and N,N‐Dimethylaminoethanol in rat liver

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Abstract
The contents of phosphatidyl N‐methyl‐and N,N‐dimethylaminoethanol were determined in the liver of rats injected with (Me‐C14) methionine. Total phospholipids were extracted from aliquots of the liver and fractionated by two‐dimensional thin layer chromatography after addition of carrier phosphatidyl‐N‐methyl‐and N,N‐dimethylaminoethanol. The radioactivity present in the two phosphatide spots was determined and used to calculated total disintegrations per min/100 g body wt. The remainder of the livers was pooled, and total phospholipids were isolated and subjected to acid hydrolysis. N‐methyl‐ and N,N‐dimethylaminoethanol were purified by thin layer chromatography, and their specific activity was determined after quantitation by gas liquid chromatography and radioactivity measurement. The liver contents of phosphatidyl N‐methyl‐ and N,N‐dimethylaminoethanol were determined by dividing disintegrations per min/100 g body wt by the specific activity of N‐methyl‐ or N,N‐dimethylaminoethanol.