Ultraviolet Determination of the Insect Repellent Diethyltoluamide

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Abstract
A rapid sensitive (1 p.p.m.) ultraviolet method of analysis has been developed and successfully applied to the determination of repellent in cloth and on glass plates. The absorbance of a sample in ethanol is read on a spectrophotometer at 230 mµ with the slit width maintained constant. The concentration is determined from a calibration curve relating absorbance to concentration. Absorption spectra of the purified ortho, meta, and para isomers have no peaks, but at constant slit width their absorbances at 230 mµ, obey Beer’s Law. Insufficient differences in the spectra of the three isomers precluded a satisfactory mathematical determination of the individual isomers.

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